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Structure analyses reveal a regulated oligomerization mechanism of the PlexinD1/GIPC/myosin VI complex

Structure analyses reveal a regulated oligomerization mechanism of the PlexinD1/GIPC/myosin VI complexThe GIPC family adaptor proteins mediate endocytosis by tethering cargo proteins to the myosin VI motor. The structural mechanisms for the GIPC/cargo and GIPC/myosin VI interactions remained unclear. PlexinD1, a transmembrane receptor that regulates neuronal and cardiovascular development, is a cargo of GIPCs. GIPC-mediated endocytic trafficking regulates PlexinD1 signaling. Here, we unravel the mechanisms of the interactions among PlexinD1, GIPCs and myosin VI by a series of crystal structures of these proteins in apo or bound states. GIPC1 forms a domain-swapped dimer in an autoinhibited conformation that hinders binding of both PlexinD1 and myosin VI. PlexinD1 binding to GIPC1 releases the autoinhibition, promoting its interaction with myosin VI. GIPCs and myosin VI interact through two distinct interfaces and form an open-ended alternating array. Our data support that this alternating array underlies the oligomerization of the GIPC/Myosin VI complexes in solution and cells.

Elife. 2017 May 24;6. pii: e27322.
doi: 10.7554/eLife.27322.

 

© 2017 eLife Sciences Publications Ltd.

  Shang G, Brautigam CA, Chen R, Lu D, Torres-Vázquez J, Zhang X.

Another SBC Highlight:

A highly potent extended half-life antibody as a potential RSV vaccine surrogate for all infants

A highly potent extended half-life antibody as a potential RSV vaccine surrogate for all infants

 

 

Sci Transl Med. 2017 May 3;9(388).
pii: eaaj1928.
doi: 10.1126/scitranslmed.aaj1928.

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When completing your Experimental Safety Approval Form (ESAF), please include the following in your experimental description:

 

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A robot is available for use at beamline 19-BM. The sample dewar accommodates ten Unipuck magazines; pins must be 18mm in length and use either SSRL or ALS bases. Experienced users may now request remote access to 19-BM when applying for beamtime.

 

 

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The on-line SBC Beamtime Request System allows users to view available beamtime on 19-ID and 19-BM, and to submit a request for rapid beamtime allocation.

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Current Beamline Statistics:

APS PDB Depositions from BioSync
SBC Sector 19 2015 2016 2017 TOTAL
ID BM ID BM ID BM
PDB Depositions 259 32 227 29 146 24 5159

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Publications Total
SBC-CAT Publication List: 19-ID 1545
SBC-CAT Publication List: 19-BM 540
SBC-CAT Publication List: ALL
APS Publications Database

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January 11-12, 2018

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July 09-12, 2018

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