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Beamtime on the 19ID and 19BM beamlines is available to the research community via a peer reviewed proposal system. SBC receives support from the U. S. Department of Energy, Office of Biological and Environmental Research.

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APS PDB Depositions from BioSynch
 SBC Current Beamline Statistics

SBC
Sector
19

2012
2013
2014
Total
ID
BM
ID
BM
ID
BM
ID & BM
PDB Deposits:
307
55
217
42
26
5
4163
73
22
70
12
2
2
1420
 

actor robot available for use on 19ID9ID

Remote Data Collection on 19ID for experienced users

A fully motorized Mini-beam device for use on 19ID

•19BM upgraded and available for immediate beamtime  

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INTERNATIONAL YEAR OF CRYSTALLOGRAPHY (IYCr)

The United Nations Educational, Scientific and Cultural Organization (UNESCO) and the International Union of Crystallography (IUCr) commemorates the centennial of X-ray diffraction and declares 2014 the International Year of Crystallography.

 

lessening x-ray damage is healthy for protein discovery data

 

Newly ID'd protein provides tArget for antibiotic-resistant hospital bacterium

 

Structural Biology Reveals the Secrets of Disease

 

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Sensor domain of histidine kinase KinB of Pseudomonas - a helix-swapped dimer.

Tan K, Chhor G, Binkowski TA, Jedrzejczak RP, Makowska-Grzyska M, Joachimiak A. J. BIOL CHEM. 2014; (Epub ahead of print)


The overproduction of polysaccharide alginate is responsible for the formation of mucus in the lungs of cystic fibrosis (CF) patients. HK KinB of the KinB-AlgB TCS in Pseudomonas aeruginosa acts as a negative regulator of alginate biosynthesis. The modular architecture of KinB is similar to other HKs. However, its periplasmic signal SD is unique and is found only in the Pseudomonas genus. Here, we present the first crystal structures of the KinB-SD. The domain is a dimer in solution and in the crystal it shows an atypical dimer of a helix-swapped four-helix bundle. A positively charged cavity is formed on the dimer interface and involves several strictly conserved residues, including R60. A phosphate anion is bound asymmetrically in one of the structures. In silico docking identified several monophosphorylated sugars, including β-D-fructose-6-phosphate and [beta]-D-mannose-6-phosphate, a precursor and an intermediate of alginate synthesis, respectively, as potential KinB ligands. Ligand binding was confirmed experimentally. Conformational transition from a symmetric to asymmetric structure and decreasing dimer stability caused by ligand binding may be a part of the signal transduction mechanism of the KinB-AlgB TCS.

 

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