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Structure of the active form of human origin recognition complex and its ATPase motor module

Structure of the active form of human origin recognition complex and its ATPase motor moduleBinding of the Origin Recognition Complex (ORC) to origins of replication marks the first step in the initiation of replication of the genome in all eukaryotic cells. Here, we report the structure of the active form of human ORC determined by X-ray crystallography and cryo-electron microscopy. The complex is composed of an ORC1/4/5 motor module lobe in an organization reminiscent of the DNA polymerase clamp loader complexes. A second lobe contains the ORC2/3 subunits. The complex is organized as a double-layered shallow corkscrew, with the AAA+ and AAA+-like domains forming one layer, and the winged-helix domains (WHDs) forming a top layer. CDC6 fits easily between ORC1 and ORC2, completing the ring and the DNA-binding channel, forming an additional ATP hydrolysis site. Analysis of the ATPase activity of the complex provides a basis for understanding ORC activity as well as molecular defects observed in Meier-Gorlin Syndrome mutations.

Elife. 2017 Jan 23;6. pii: e20818.
doi: 10.7554/eLife.20818.

 

© 2017 eLife Sciences Publications Ltd.

  Tocilj A, On KF, Yuan Z, Sun J, Elkayam E, Li H, Stillman B, Joshua-Tor L.

Another SBC Highlight:

Inhibition of RAS function through targeting an allosteric regulatory site
Inhibition of RAS function through targeting an allosteric regulatory site

 

 

Nat Chem Biol. 2017 Jan;13(1):62-68.
doi: 10.1038/nchembio.2231. Epub 2016 Nov 7.

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SBC Announcements:

 

19-ID Pilatus Detector

 

SBC has implemented a Pilatus 3 X 6M detector into the User Program at beamline 19-ID. Data collected by our users has been of outstanding quality.

 

 

19-BM Robotic sample mounter available

 

A robot is available for use at beamline 19-BM. The sample dewar accommodates ten Unipuck magazines; pins must be 18mm in length and use either SSRL or ALS bases. Experienced users may now request remote access to 19-BM when applying for beamtime.

 

 

SBC Staff Hosting Changes

 

SBC staff are now available for User Support between the hours of 8:00am and 10:pm.

 

 

 

ALSO: Please Be Aware...!

 

APS requests the Experimental Safety Approval Form (ESAF) be submitted at least seven days prior to your scheduled beamtime.

 

Failure to submit your ESAF in advance may result in delaying the beginning of your experiment.

 

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If you have any suggestions or comments, please feel free to e-mail us.

 

Rapid Access Beamtime Available:

SBC Beamtime Request System

The on-line SBC Beamtime Request System allows users to view available beamtime on 19-ID and 19-BM, and to submit a request for rapid beamtime allocation.

Beamtime is available to the research community via a peer reviewed proposal system; it is supported by the U.S. Department of Energy, Office of Biological and Environmental Research.

Current Beamline Statistics:

APS PDB Depositions from BioSync
SBC Sector 19 2015 2016 2017 TOTAL
ID BM ID BM ID BM
PDB Depositions 263 32 161 26 28 6 4954

APS Publications Database:

Publications Total
SBC-CAT Publication List: 19-ID 1468
SBC-CAT Publication List: 19-BM 528
SBC-CAT Publication List: ALL
APS Publications Database

MCSG / APCF Announcements:

 

The Midwest Center for Structural Genomics (MCSG) is accepting applications for access to the MCSG User Resource.

 

MCSG's structure determination platform is well established, and combines technologies, robotics and expertise for gene cloning, protein production, and crystallization, as well as biochemical and biophysical characterization.

 

For further information, please contact Andrzej Joachimiak.

 

Future Meetings:

 

XXIII West Coast Protein Crystallography Workshop

March 19-22, 2017

Pacific Grove; California, USA

 

2017 APS/CNM User Meeting

May 8-11, 2017

Advanced Photon Source; Chicago; Illinois, USA

 

2017 Annual Meeting of the American Crystallographic Association

May 26-30, 2017

New Orleans; Louisiana, USA

 

SBC Beamline Phone Numbers:

Phone Location Number
19-ID Beamline Control Station 630-252-9823
19-ID Beamline Cordless Phone 630-252-0569
19-ID Beamline Inside D-Hutch 630-252-1819
 
19-BM Beamline Control Station 630-252-9833
19-BM Beamline Cordless Phone 630-252-0568
19-BM Beamline Inside D-Hutch 630-252-1719