Structural Biology Center
Home About Us Facilities User Program Resources Research Links Contact Us

Quick Links:

Home Pages:


Structure analyses reveal a regulated oligomerization mechanism of the PlexinD1/GIPC/myosin VI complex

Structure analyses reveal a regulated oligomerization mechanism of the PlexinD1/GIPC/myosin VI complexThe GIPC family adaptor proteins mediate endocytosis by tethering cargo proteins to the myosin VI motor. The structural mechanisms for the GIPC/cargo and GIPC/myosin VI interactions remained unclear. PlexinD1, a transmembrane receptor that regulates neuronal and cardiovascular development, is a cargo of GIPCs. GIPC-mediated endocytic trafficking regulates PlexinD1 signaling. Here, we unravel the mechanisms of the interactions among PlexinD1, GIPCs and myosin VI by a series of crystal structures of these proteins in apo or bound states. GIPC1 forms a domain-swapped dimer in an autoinhibited conformation that hinders binding of both PlexinD1 and myosin VI. PlexinD1 binding to GIPC1 releases the autoinhibition, promoting its interaction with myosin VI. GIPCs and myosin VI interact through two distinct interfaces and form an open-ended alternating array. Our data support that this alternating array underlies the oligomerization of the GIPC/Myosin VI complexes in solution and cells.

Elife. 2017 May 24;6. pii: e27322.
doi: 10.7554/eLife.27322.


© 2017 eLife Sciences Publications Ltd.

  Shang G, Brautigam CA, Chen R, Lu D, Torres-Vázquez J, Zhang X.

Another SBC Highlight:

A highly potent extended half-life antibody as a potential RSV vaccine surrogate for all infants

A highly potent extended half-life antibody as a potential RSV vaccine surrogate for all infants



Sci Transl Med. 2017 May 3;9(388).
pii: eaaj1928.
doi: 10.1126/scitranslmed.aaj1928.

Previous SBC Highlights:


Highlights featured previously on our web-page


ANL and APS Highlights:


Highlights featured by either ANL or APS


SBC Announcements:


ESAF Requirements:


When completing your Experimental Safety Approval Form (ESAF), please include the following in your experimental description:


APS requests the Experimental Safety Approval Form be submitted at least seven days prior to your scheduled beamtime.


Failure to submit your ESAF according to this schedule may result in delaying the beginning of your experiment.



19-BM Robotic sample mounter available


A robot is available for use at beamline 19-BM. The sample dewar accommodates ten Unipuck magazines; pins must be 18mm in length and use either SSRL or ALS bases. Experienced users may now request remote access to 19-BM when applying for beamtime.



SBC Staff Hosting Changes


SBC staff are now available for User Support between the hours of 8:00am and 10:pm.


Web Site:


Web Site Suggestions?


If you have any suggestions or comments, please feel free to e-mail us.


Rapid Access Beamtime Available:

SBC Beamtime Request System

The on-line SBC Beamtime Request System allows users to view available beamtime on 19-ID and 19-BM, and to submit a request for rapid beamtime allocation.

Beamtime is available to the research community via a peer reviewed proposal system; it is supported by the U.S. Department of Energy, Office of Biological and Environmental Research.

Current Beamline Statistics:

APS PDB Depositions from BioSync
SBC Sector 19 2015 2016 2017 TOTAL
PDB Depositions 259 32 227 29 146 24 5159

APS Publications Database:

Publications Total
SBC-CAT Publication List: 19-ID 1545
SBC-CAT Publication List: 19-BM 540
SBC-CAT Publication List: ALL
APS Publications Database

MCSG / APCF Announcements:


Access to the Advanced Protein Characterization Facility (APCF), housing the Midwest Center for Structural Genomics (MCSG), requires successful completion of an ANL Guest/Joint Appointee application, procurement of an Argonne domain account, and currently valid requisite training for building #446 and ANL.


MCSG's structure determination platform is well established, and combines technologies, robotics and expertise for gene cloning, protein production, and crystallization, as well as biochemical and biophysical characterization.


For further information, please contact Andrzej Joachimiak.


Future Meetings:


CCP4 Study Weekend

January 11-12, 2018

Nottingham; East Midlands, England; UK


American Crystallographic Association: 2018

July 20-24, 2018

Toronto; Ontario, Canada


Protein Society's 32nd Annual Symposium

July 09-12, 2018

Boston; Massachusetts, USA


SBC Beamline Phone Numbers:

Phone Location Number
19-ID Beamline Control Station 630-252-9823
19-ID Beamline Cordless Phone 630-252-0569
19-ID Beamline Inside D-Hutch 630-252-1819
19-BM Beamline Control Station 630-252-9833
19-BM Beamline Cordless Phone 630-252-0568
19-BM Beamline Inside D-Hutch 630-252-1719