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The CDI toxin of Yersinia kristensenii is a novel bacterial member of the RNase A superfamily

The CDI toxin of Yersinia kristensenii is a novel bacterial member of the RNase A superfamilyContact-dependent growth inhibition (CDI) is an important mechanism of inter-bacterial competition found in many Gram-negative pathogens. CDI+ cells express cell-surface CdiA proteins that bind neighboring bacteria and deliver C-terminal toxin domains (CdiA-CT) to inhibit target-cell growth. CDI+ bacteria also produce CdiI immunity proteins, which specifically neutralize cognate CdiA-CT toxins to prevent self-inhibition. Here, we present the crystal structure of the CdiA-CT/CdiIYkris complex from Yersinia kristensenii ATCC 33638. CdiA-CTYkris adopts the same fold as angiogenin and other RNase A paralogs, but the toxin does not share sequence similarity with these nucleases and lacks the characteristic disulfide bonds of the superfamily. Consistent with the structural homology, CdiA-CTYkris has potent RNase activity in vitro and in vivo. Structure-guided mutagenesis reveals that His175, Arg186, Thr276 and Tyr278 contribute to CdiA-CTYkris activity, suggesting that these residues participate in substrate binding and/or catalysis. CdiIYkris binds directly over the putative active site and likely neutralizes toxicity by blocking access to RNA substrates. Significantly, CdiA-CTYkris is the first non-vertebrate protein found to possess the RNase A superfamily fold, and homologs of this toxin are associated with secretion systems in many Gram-negative and Gram-positive bacteria. These observations suggest that RNase A-like toxins are commonly deployed in inter-bacterial competition.

Nucleic Acids Res. 2017 May 19;45(9):5013-5025.
doi: 10.1093/nar/gkx230.

 

© 2017 Oxford University Press.

  Batot G, Michalska K, Ekberg G, Irimpan EM, Joachimiak G, Jedrzejczak R, Babnigg G, Hayes CS, Joachimiak A, Goulding CW.

Another SBC Highlight:

Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis

 

Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis

 

 

Nat Commun. 2017 Jun 8;8:15576.
doi: 10.1038/ncomms15576.

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When completing your Experimental Safety Approval Form (ESAF), please include the following in your experimental description:

 

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The on-line SBC Beamtime Request System allows users to view available beamtime on 19-ID and 19-BM, and to submit a request for rapid beamtime allocation.

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Current Beamline Statistics:

APS PDB Depositions from BioSync
SBC Sector 19 2015 2016 2017 TOTAL
ID BM ID BM ID BM
PDB Depositions 259 32 205 29 93 14 5069

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Publications Total
SBC-CAT Publication List: 19-ID 1512
SBC-CAT Publication List: 19-BM 533
SBC-CAT Publication List: ALL
APS Publications Database

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Protein Society's 31st Annual Symposium

July 24-27, 2017

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