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New aminopeptidase from "microbial dark matter" archaeon

BAP_monomer Marine sediments host a large population of diverse, heterotrophic, uncultured microorganisms with unknown physiologies that control carbon flow through organic matter decomposition.

Recently, single-cell genomics uncovered new key players in these processes, such as the miscellaneous crenarchaeotal group.

These widespread archaea encode putative intra- and extracellular proteases for the degradation of detrital proteins present in sediments.

Here, we show that one of these enzymes is a self-compartmentalizing tetrameric amino-peptidase with a preference for cysteine and hydrophobic residues at the N terminus of the hydrolyzed peptide.

The ability to perform detailed characterizations of enzymes from native subsurface microorganisms, without requiring that those organisms first be grown in pure culture, holds great promise for understanding key carbon transformations in the environment as well as identifying new enzymes for biomedical and biotechnological applications.

FASEB Journal; published online June 10, 2015. fj.15-272906.

Karolina Michalska, Andrew D. Steen, Gekleng Chhor, Michael Endres, Austen T. Webber, Jordan Bird, Karen G. Lloyd, and Andrzej Joachimiak.

ANL Feature Story:

HNF-4alpha
Argonne Researchers Uncover Structure of Protein Implicated in Diabetes

Hepatocyte nuclear factor 4α (HNF-4α), plays a critical role by binding to specific DNA sequences and regulating the production of a number of key proteins for normal cellular processes. Some of its mutations have been linked to maturity-onset diabetes, kidney failure and metabolic syndrome.

 

Nature. 2013;495(7441):394–395

Meetings:

 

2015 Annual Meeting of the American Crystallographic Association

July 25–29 (Sheraton Downtown Hotel; Philadelphia, PA)

 

SBC Announcements:

 

Web Site Suggestions?

 

It's a whole new web site, with different organization. If you have any suggestions or comments, please feel free to e-mail us.

 

ANL Announcements:

 

ANL Site Access Changes

 

Effective June 1, 2015, Argonne National Laboratory will implement the enforcement of the REAL ID Act, affecting site access for US citizens from Arizona, Louisiana, Minnesota, New Hampshire, and New York. A driver's license will NOT be an acceptable form of identification.

Rapid Access Beamtime Available:

SBC Beamtime Request System

The on-line SBC Beamtime Request System allows users to view available beamtime on 19-ID and 19-BM, and to submit a request for rapid beamtime allocation.

Beamtime is available to the research community via a peer reviewed proposal system; it is supported by the U.S. Department of Energy, Office of Biological and Environmental Research.

Current Beamline Statistics:

APS PDB Depositions from BioSync
SBC Sector 19 2013 2014 2015 TOTAL
ID BM ID BM ID BM
PDB Depositions 238 49 245 31 40 6 4464

APS Publications Database:

Publications Total
SBC-CAT Publication List: 19-ID 1457
SBC-CAT Publication List: 19-BM 558
SBC-CAT Publication List: ALL 1809
ANL Publications Database

MCSG / APCF Announcements:

 

Beginning August 01, 2015 the Midwest Center for Structural Genomics (MCSG) will start accepting applications for access to the MCSG User Resource.

 

MCSG's structure determination platform is well established, and combines technologies, robotics and expertise for gene cloning, protein production, and crystallization, as well as biochemical and biophysical characterization.

 

For further information, please contact Andrzej Joachimiak.