Biochemistry Laboratory
Laboratory space is available for the use of SBC Users. Access is gained via the User Badge and a key-card reader mounted at one of the laboratory doors. Please note that only BioSafety Level One (BSL-1) experiments will typically be approved. A few projects requiring BSL-2 facilities may be allowed at SBC beamlines, pending further review. Projects requiring either BSL-3 or BSL-4 facilities cannot be conducted at the SBC facilities. Information regarding these classification definitions may be found at (link): Biosafety in Microbiological and Biomedical Laboratories.
A small workbench area will be available for use during data collection time. Due to the presence of other scheduled Users, access to the laboratory may only be available immediately before and after the assigned data collection time.
A walk-in, +5° C cold room is available. One biochemistry workbench area is available in the cold room, and is shared by all 19ID and 19BM Users.
One fume hood is present. Work involving either freezing of crystals in liquid-propane or heavy-atom compounds must be performed in this fume hood.
Microscopes are available for viewing crystals. Two microscopes are available for 19ID; one in the biochemistry laboratory, one inside the beamline experimental hutch. Two additional, analogous microscopes are available for beamline 19BM. A fifth microscope is available in the cold room (+5° C). Preferential access to these microscopes is given to User groups actively collecting data at the beamlines. All microscopes are locked down in position, and may not be moved.
Limited laboratory equipment is available for general use by all SBC Users. Although SBC staff try to maintain equipment in good working order, please be aware that heavy use may lead to repairs and unavailability of the equipment during your assigned beamtime. As always, if you feel the success of your experiment will depend upon the availability of consumable items, please bring your own supplies with you from your home institution.
Equipment presently available:
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Consumables that may be available, depending upon funding and time constraints: |
Equipment that is NOT available:
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Dewars; for freezing crystals and transferring frozen crystals |
Racks, tweezers, and canes; for assisting
in freezing crystals and transferring frozen crystals |
Hampton Research supplies
- of any sort! |
Analytical Balance; 0.0 – 10.0 g range |
Disposable tips; 10µl, 200µl, 1000µl |
Loops, pins, crystal-mounting tools |
Pan Balance; 0.0 – 200.0 g range |
Glass microscope slides |
Chemicals |
Microfuge; +5° C; single speed, approximately 10,000 rpm; NOT suitable for concentration-type membranes |
Microfuge tubes; 0.50ml, 1.50ml
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Bunsen burners; or any other source of open flame |
Vortex mixer; +5 ° C |
pH paper; few strips |
Aspirators; or any other source of biochemistry laboratory vacuum |
pH meter |
Magnetic stirrer bars; few sizes |
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Hot plate; with stirrer function |
Aluminum foil |
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Stirrer plate; no hot-plate function |
Plastic wrap |
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Milli-Q water; limited quantities |
Ethanol; 95% |
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Ice machine; access to APS
supplied ice (+0 ° C) |
Acetone |
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Paper tape |
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Scissors |
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All samples transported to SBC must be packaged and shipped according to Department of Transport (DOT) regulations.
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