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RCC2 is a novel p53 target in suppressing metastasis

RCC2 is a novel p53 target in suppressing metastasisRCC2 (also known as TD60) is a highly conserved protein involved in prognosis in colorectal cancer. However, its relationship with tumor development is less understood. Here we demonstrate a signaling pathway defining regulation of RCC2 and its functions in tumor progression. We report that p53 is a transcriptional regulator of RCC2 that acts through its binding to a palindromic motif in the RCC2 promoter. RCC2 physically interacts and deactivates a small GTPase Rac1 that is known to be involved in metastasis. We solved a high-resolution crystal structure of RCC2 and revealed one RCC1-like domain with a unique β-hairpin that is requisite for RCC2 interaction with Rac1. p53 or RCC2 deficiency leads to activation of Rac1 and deterioration of extracellular matrix sensing (haptotaxis) of surface-bound gradients. Ectopic expression of RCC2 restores directional migration in p53-null cells. Our results demonstrate that p53 and RCC2 signaling is important for regulation of cell migration and suppression of metastasis. We propose that the p53/RCC2/Rac1 axis is a potential target for cancer therapy.

Oncogene. 2018 Jan 4;37(1):8-17.
doi: 10.1038/onc.2017.306. Epub 2017 Sep 4.


© 2018 Macmillan Publishers Limited.

  Song C, Liang L, Jin Y, Li Y, Liu Y, Guo L, Wu C, Yun CH, Yin Y.

Another SBC Highlight:

DNA Sequence Recognition of Human CXXC Domains and Their Structural Determinants

Structure-guided design of an Hsp90β N-terminal isoform-selective inhibitor



Nat Commun. 2018 Jan 30;9(1):425.
doi: 10.1038/s41467-017-02013-1.

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New Oxford Cryostream 800 Series now available on both 19-ID and 19-BM.


Rigaku Actor Robot, with heated end effector, upgraded on 19-ID.


A sample-mounting robot is available for use at beamline 19-BM. The dewar accommodates ten Unipuck magazines; pins must be 18mm in length and use either SSRL or ALS bases. Experienced users may now request remote access to 19-BM when applying for beamtime.



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When completing your Experimental Safety Approval Form (ESAF), please include the following in your experimental description:


APS requests the Experimental Safety Approval Form be submitted at least seven days prior to your scheduled beamtime.


Failure to submit your ESAF according to this schedule may result in delaying the beginning of your experiment.



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The on-line SBC Beamtime Request System allows users to view available beamtime on 19-ID and 19-BM, and to submit a request for rapid beamtime allocation.

Beamtime is available to the research community via a peer reviewed proposal system; it is supported by the U.S. Department of Energy, Office of Biological and Environmental Research.

Current Beamline Statistics:

APS PDB Depositions from BioSync
SBC Sector 19 2016 2017 2018 TOTAL
PDB Depositions 232 29 192 37 09 06 5237

APS Publications Database:

Publications Total
SBC-CAT Publication List: 19-ID 1568
SBC-CAT Publication List: 19-BM 545
SBC-CAT Publication List: ALL
APS Publications Database

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Access to the Advanced Protein Characterization Facility (APCF), housing the Midwest Center for Structural Genomics (MCSG), requires successful completion of an ANL Guest/Joint Appointee application, procurement of an Argonne domain account, and currently valid requisite training for building #446 and ANL.


MCSG's structure determination platform is well established, and combines technologies, robotics and expertise for gene cloning, protein production, and crystallization, as well as biochemical and biophysical characterization.


For further information, please contact Andrzej Joachimiak.


Future Meetings:


APS/CNM Users Meeting

May 7-10, 2018

Advanced Photon Source; Chicago, USA


Protein Society's 32nd Annual Symposium

July 09-12, 2018

Boston; Massachusetts, USA


American Crystallographic Association: 2018

July 20-24, 2018

Toronto; Ontario, Canada


SBC Beamline Phone Numbers:

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19-ID Beamline Control Station 630-252-9823
19-ID Beamline Cordless Phone 630-252-0569
19-ID Beamline Inside D-Hutch 630-252-1819
19-BM Beamline Control Station 630-252-9833
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19-BM Beamline Inside D-Hutch 630-252-1719