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Molecular basis for branched steviol glucoside biosynthesis

 

Lee SG, Salomon E, Yu O, Jez JM.

 

Proc Natl Acad Sci U S A. 2019, Jun 10. pii: 201902104. doi: 10.1073/pnas.1902104116

RCC2 is a novel p53 target in suppressing metastasisThe naturally occurring noncaloric sweetener stevia is a plant natural product consisting of a core terpene structure decorated with a specific pattern of glucose molecules, including a branched three-sugar unit. Stevia and other related molecules are being explored as noncaloric dietary sweeteners because they can help maintain the health of diabetic, phenylketonuric, and obese patients. Here, we describe the three-dimensional structure of the plant enzyme (UGT76G1) that forms the branched group of sugars that defines the stevia molecule and is critical for its high-intensity sweetness. Understanding how this enzyme forms this chemical group provides insight on how the stevia plant makes this sweetener and suggests how to alter the protein to generate new versions of the noncaloric sweetener.

 

 

 

In the News:

Structuring sweetness: What makes Stevia 200 times sweeter than sugar

 

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SBC Announcements:

Job Opening: Postdoctoral Appointee

Argonne National Laboratory seeks a postdoctoral researcher to join the Structural Biology Center (SBC) at the Advanced Photon Source (APS) to develop methods in crystallography and other techniques. Building on our expertise in high resolution de novo structure determination, serial crystallography and a long history of cutting edge experiments, we would like to bring the next generation of experiments to the SBC general user program. The postdoctoral fellow will develop hardware and software to analyze molecular changes of structures as diffraction data is collected and processed autonomously, will create the experimental scope, source samples, collect and analyze data.

 

Requisition # 406434

Apply Here

 

 

Student Training:

SBC offers applications for remote faculty supervised training of students in remote data collection on 19BM beamline.  SBC can provide standard protein crystals (lysozyme, thaumatin) for data collection. SBC resources for data collection, processing and structure determination will be available. The SBC staff will assist in training. This training will help the next generation of synchrotron light source users and scientists learn software and strategies for data collection, data processing, and structure determination.  Please contact mficner@anl.gov for more information.

 

 

Upgraded ADSC 315r Detector on 19-BM with double the area size and atomic resolution.

 

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Rigaku Actor Robot, with heated end effector, upgraded on 19-ID.

 

Sample-mounting robots available at

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When completing your Experimental Safety Approval Form (ESAF), please include the following in your experimental description:

 

APS requests the Experimental Safety Approval Form be submitted at least seven days prior to your scheduled beamtime.

 

Failure to submit your ESAF according to this schedule may result in delaying the beginning of your experiment.

 

 

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The on-line SBC Beamtime Request System allows users to view available beamtime on 19-ID and 19-BM, and to submit a request for rapid beamtime allocation.

Beamtime is available to the research community via a peer reviewed proposal system; it is supported by the U.S. Department of Energy, Office of Biological and Environmental Research.

Current Beamline Statistics:

APS PDB Depositions from BioSync
SBC Sector 19 2017 2018 2019 TOTAL
ID BM ID BM ID BM
PDB Depositions 263 52 148 38 53 13 5551

APS Publications Database:

Publications Total
SBC-CAT Publication List: 19-ID 1684
SBC-CAT Publication List: 19-BM 578
APS Publications Database

SBC Acknowlegement:

Results shown in this report are derived from work performed at Argonne National Laboratory (ANL), Structural Biology Center (SBC) at the Advanced Photon Source (APS), under U.S. Department of Energy, Office of Biological and Environmental Research contract DE-AC02-06CH11357."

 

 

Use of the APCF:

APS Sector 84 at the APCF will operate the high-throughput protein structure determination and characterization pipeline as a resource for Biology Community. The structure determination platform is well established and it combines technologies, robotics and expertise for gene cloning, protein production, crystallization, and biochemical and biophysical characterization. Data collection and structure determination will be done using SBC and other MX beamlines at the APS. The APCF maintains a bank of microbial genomic DNA, protein expression vectors and clones, variety of proteomics and computational software tools and project databases. The APCF will host and train visiting scientists.

 

To apply: Sumbit an ESAF for Sector 84.

 

APCF Acknowlegement: Access to Sector 84 laboratories at the Advanced Protein Characterization Facility (APCF) was possible through funding provided by NIH grant GM115586 and DOE contract DE-AC02-06CH11357.

 

For more information please contact

Michelle Radford or Paula Bulaon.

 

 

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