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Phosphorylation of spore coat proteins by a family of atypical protein kinases

Bile salt receptor complex activates a pathogenic type III secretion systemBile is an important component of the human gastrointestinal tract with an essential role in food absorption and antimicrobial activities. Enteric bacterial pathogens have developed strategies to sense bile as an environmental cue to regulate virulence genes during infection. We discovered that Vibrio parahaemolyticus VtrC, along with VtrA and VtrB, are required for activating the virulence type III secretion system 2 in response to bile salts. The VtrA/VtrC complex activates VtrB in the presence of bile salts. The crystal structure of the periplasmic domains of the VtrA/VtrC heterodimer reveals a b-barrel with a hydrophobic inner chamber. A co-crystal structure of VtrA/ VtrC with bile salt, along with biophysical and mutational analysis, demonstrates that the hydrophobic chamber binds bile salts and activates the virulence network. As part of a family of conserved signaling receptors, VtrA/VtrC provides structural and functional insights into the evolutionarily conserved mechanism used by bacteria to sense their environment.

Elife. 2016 Jul 5;5. pii: e15718.
doi: 10.7554/eLife.15718.

eLife Sciences Publications, Ltd.

© 2016 eLife Sciences Publications Ltd.

  Li P, Rivera-Cancel G, Kinch LN, Salomon D, Tomchick DR, Grishin NV, Orth K.

Another SBC Highlight:

Phosphorylation of spore coat proteins by a family of atypical protein kinases
Phosphorylation of spore coat proteins by a family of atypical protein kinases.

The modification of proteins by phosphorylation occurs in all life forms and is catalyzed by a large superfamily of enzymes known as protein kinases. We recently discovered a family of secretory pathway kinases that phosphorylate extracellular proteins.

 

Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):E3482-91.
doi: 10.1073/pnas.1605917113.
Epub 2016 May 16.

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SBC Announcements:

 

19-ID Pilatus Detector

SBC is pleased to announce the introduction of a Pilatus 3 X 6M detector into the User Program at beamline 19-ID.
Data collected by our users has been of outstanding quality.

 

 

 

19-BM Rebotic sample mounter available

A robot is now available for use at beamline 19-BM. The sample dewar accommodates ten Unipuck magazines; pins must be 18mm in length and use either SSRL or ALS bases. Experienced users may now request remote access to 19-BM when applying for beamtime.

 

 

 

ALSO: Please Be Aware...!

 

APS requests the Experimental Safety Approval Form (ESAF) be submitted at least seven days prior to your scheduled beamtime.

 

Failure to submit your ESAF in advance may result in delaying the beginning of your experiment.

 

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If you have any suggestions or comments, please feel free to e-mail us.

 

Rapid Access Beamtime Available:

SBC Beamtime Request System

The on-line SBC Beamtime Request System allows users to view available beamtime on 19-ID and 19-BM, and to submit a request for rapid beamtime allocation.

Beamtime is available to the research community via a peer reviewed proposal system; it is supported by the U.S. Department of Energy, Office of Biological and Environmental Research.

Current Beamline Statistics:

APS PDB Depositions from BioSync
SBC Sector 19 2014 2015 2016 TOTAL
ID BM ID BM ID BM
PDB Depositions 263 36 237 30 89 13 4807

APS Publications Database:

Publications Total
SBC-CAT Publication List: 19-ID 1423
SBC-CAT Publication List: 19-BM 520
SBC-CAT Publication List: ALL
APS Publications Database

MCSG / APCF Announcements:

 

Beginning August 01, 2015 the Midwest Center for Structural Genomics (MCSG) will start accepting applications for access to the MCSG User Resource.

 

MCSG's structure determination platform is well established, and combines technologies, robotics and expertise for gene cloning, protein production, and crystallization, as well as biochemical and biophysical characterization.

 

For further information, please contact Andrzej Joachimiak.

 

Future Meetings:

 

X-Ray Methods in Structural Biology

October 10-25, 2016

Cold Spring Harbor Laboratory; Cold Spring Harbor, NY

 

De-Mystifying X-ray Data Processing in Macromolecular Crystallography

November 14-15, 2016

Charles Darwin House; London, UK

 

DLS-CCP4 Data Collection and Structure Solution Workshop

December 13-20, 2016

Diamond Light Source; Oxfordshire, UK

 

SBC Beamline Phone Numbers:

Phone Location Number
19-ID Beamline Control Station 630-252-9823
19-ID Beamline Cordless Phone 630-252-0569
19-ID Beamline Inside D-Hutch 630-252-1819
 
19-BM Beamline Control Station 630-252-9833
19-BM Beamline Cordless Phone 630-252-0568
19-BM Beamline Inside D-Hutch 630-252-1719